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Western blot analysis of various lysates using MED4 Rabbit mAb (A0623) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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Immunohistochemistry analysis of paraffin-embeddedHuman breast cancer tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman breast tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman esophagus tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman colon tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedRat colon tissue usingMED4 Rabbit mAb(A0623) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |