Matrin 3 Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	Matrin 3 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A1027
Hersteller Artikelnummer:	A1027
Alternativnummer:	ABB-A1027-20UL,ABB-A1027-100UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	MPD2, ALS21, VCPDM, Matrin 3

This gene encodes a nuclear matrix protein, which is proposed to stabilize certain messenger RNA species. Mutations of this gene are associated with distal myopathy 2, which often includes vocal cord and pharyngeal weakness. Alternatively spliced transcript variants, including read-through transcripts composed of the upstream small nucleolar RNA host gene 4 (non-protein coding) and matrin 3 gene sequence, have been identified. Pseudogenes of this gene are located on chromosomes 1 and X.

Klonalität:	Monoclonal
Molekulargewicht:	95kDa
NCBI:	9782
UniProt:	P43243
Reinheit:	Affinity purification
Target-Kategorie:	MATR3

Application Verdünnung:	WB,1:500 - 1:1000\|IHC-P,1:50 - 1:200\|IF/ICC,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding. Shipping: Ice Bag

	Western blot analysis of various lysates using Matrin 3 Rabbit mAb (A1027) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Western blot analysis of lysates from Rat brain, using Matrin 3 Rabbit mAb (A1027) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry analysis of paraffin-embedded Rat ovary using Matrin 3 Rabbit mAb (A1027) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human brain using Matrin 3 Rabbit mAb (A1027) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using Matrin 3 Rabbit mAb (A1027) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunofluorescence analysis of C6 cells using Matrin 3 Rabbit mAb (A1027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using Matrin 3 Rabbit mAb (A1027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using Matrin 3 Rabbit mAb (A1027) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.