Snail Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer:
ABB-A11794
- Bilder (8)
| Artikelname: | Snail Rabbit pAb, Unconjugated, Polyclonal |
| Artikelnummer: | ABB-A11794 |
| Hersteller Artikelnummer: | A11794 |
| Alternativnummer: | ABB-A11794-100UL,ABB-A11794-20UL,ABB-A11794-1000UL,ABB-A11794-500UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | SNA, SNAH, SNAIL, SLUGH2, SNAIL1, dJ710H13.1, Snail |
| The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. |
| Application Verdünnung: | WB,1:500 - 1:1000|IHC-P,1:50 - 1:200|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Cancer,Cardiovascular,Heart,Cardiogenesis. Shipping: Ice Bag |
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Western blot analysis of various lysates using Snail Rabbit pAb (A11794) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 µg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:1s. |
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Western blot analysis of lysates from NIH/3T3 cells usingSnail Rabbit pAb (A11794) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:15s. |
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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with Snail using Snail Rabbit pAb (A11794) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 20µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Western blot analysis of various lysates using Snail Rabbit pAb (A11794) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embedded Rat spleen using Snail Rabbit pAb (A11794) at dilution of 1:150 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Snail Rabbit pAb (A11794) at dilution of 1:150 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse lung using Snail Rabbit pAb (A11794) at dilution of 1:150 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining. |
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Immunofluorescence analysis of L929 cells using Snail Rabbit pAb (A11794) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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