Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:
Unconjugated
Alternative Synonym:
ARK5, NUAK1
Enables p53 binding activity and protein serine/threonine kinase activity. Involved in several processes, including protein phosphorylation, regulation of cellular senescence, and regulation of myosin-light-chain-phosphatase activity. Located in cytoplasm, microtubule cytoskeleton, and nuclear lumen.
WB,1:200 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Human,Rat. ResearchArea: Cancer,Signal Transduction,Kinase,Serine threonine kinases. Shipping: Ice Bag
Western blot analysis of lysates from rat heart, using NUAK1 Rabbit pAb (A17333) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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