Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Alternative Synonym:
CAPAM, hCAPAM, hPCIF1, C20orf67, PPP1R121, PCIF1
Enables RNA polymerase II C-terminal domain phosphoserine binding activity, S-adenosyl-L-methionine binding activity, and mRNA (2-O-methyladenosine-N6-)-methyltransferase activity. Involved in mRNA methylation, negative regulation of translation, and positive regulation of translation. Located in intercellular bridge, microtubule cytoskeleton, and nucleoplasm.
WB,1:1000 - 1:5000|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling. Shipping: Ice Bag
Western blot analysis of lysates from HeLa cells, using PCIF1 Rabbit pAb (A18347) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
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