Fluorescein Isothiocyanate/FITC Rabbit pAb, Unconjugated, Polyclonal
Artikelnummer:
ABB-A20385
- Bilder (1)
| Artikelname: | Fluorescein Isothiocyanate/FITC Rabbit pAb, Unconjugated, Polyclonal |
| Artikelnummer: | ABB-A20385 |
| Hersteller Artikelnummer: | A20385 |
| Alternativnummer: | ABB-A20385-100UL,ABB-A20385-20UL,ABB-A20385-1000UL,ABB-A20385-500UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Spezies Reaktivität: | All |
| Immunogen: | Chemical compounds corresponding to Fluorescein Isothiocyanate/FITC. |
| Konjugation: | Unconjugated |
| Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is also widely used as a hapten to label different proteins. |
| Klonalität: | Polyclonal |
| Reinheit: | Affinity purification |
| Sequenz: | FITC conjugated to KLH |
| Target-Kategorie: | FITC |
| Antibody Type: | Primary Antibody |
| Application Verdünnung: | WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Species independent. Shipping: Ice Bag |
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The FITC rabbit polyclonal antibody (A20385) are tested in Western blot against FITC conjugated Goat IgG,TRITC conjugated Goat IgG and unconjugated Goat IgG Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: Refer to figures. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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