Alpha-Fetoprotein (AFP) Rabbit mAb, Monoclonal
Artikelnummer:
ABB-A22053
- Bilder (1)
| Artikelname: | Alpha-Fetoprotein (AFP) Rabbit mAb, Monoclonal |
| Artikelnummer: | ABB-A22053 |
| Hersteller Artikelnummer: | A22053 |
| Alternativnummer: | ABB-A22053-20UL,ABB-A22053-100UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Alternative Synonym: | AFPD, FETA, HPAFP, Alpha-Fetoprotein (AFP) |
| This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
| Application Verdünnung: | WB,1:1000 - 1:5000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human. ResearchArea: Cancer,Tumor biomarkers,Cell Biology Developmental Biology,Stem Cells,Cardiovascular,Blood,Serum Proteins. Shipping: Ice Bag |
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Western blot analysis of various lysates using Alpha-Fetoprotein (AFP) Rabbit mAb (A22053) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): THP-1. Exposure time: 30s. |
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