DNA Ligase III/LIG3 Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	DNA Ligase III/LIG3 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A22136
Hersteller Artikelnummer:	A22136
Alternativnummer:	ABB-A22136-100UL,ABB-A22136-20UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	LIG2, MTDPS20, LIG3alpha, DNA Ligase III/LIG3

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Klonalität:	Monoclonal
Molekulargewicht:	113kDa
NCBI:	3980
UniProt:	P49916
Reinheit:	Affinity purification
Target-Kategorie:	LIG3

Application Verdünnung:	WB,1:1000 - 1:5000\|IHC-P,1:50 - 1:200\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling. Shipping: Ice Bag

	Western blot analysis of various lysates, using DNA Ligase III/LIG3 Rabbit mAb (A22136) at1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using DNA Ligase III/LIG3 Rabbit mAb (A22136) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.