PGAM5 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A22203
Artikelname: PGAM5 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A22203
Hersteller Artikelnummer: A22203
Alternativnummer: ABB-A22203-100UL,ABB-A22203-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: BXLBV68, PGAM5
Enables GTPase activator activity and protein serine/threonine phosphatase activity. Involved in necroptotic process. Located in mitochondrion.
Klonalität: Monoclonal
Molekulargewicht: 32kDa/28KDa
NCBI: 192111
UniProt: Q96HS1
Reinheit: Affinity purification
Target-Kategorie: PGAM5
Application Verdünnung: WB,1:20000 - 1:80000|IHC-P,1:100 - 1:500|IF/ICC,1:300 - 1:1200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Signal Transduction. Shipping: Ice Bag
Western blot analysis of various lysates, using PGAM5 Rabbit mAb (A22203) at1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using PGAM5 Rabbit mAb (A22203) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of Hep G2 cells using PGAM5 Rabbit mAb (A22203,dilution 1:300)(Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x.