Involved in regulation of mitotic cell cycle. Acts upstream of or within centrosome localization, mitotic spindle organization, and protein localization to nuclear envelope. Located in cytoplasm and nuclear body.
Western blot analysis of various lysates, using ASUN/INTS13 Rabbit mAb (A22792) at 1:70000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
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