[KD Validated] BRD2 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A23166
Artikelname: [KD Validated] BRD2 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A23166
Hersteller Artikelnummer: A23166
Alternativnummer: ABB-A23166-100UL,ABB-A23166-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: FSH, NAT, RNF3, FSRG1, RING3, FSHRG1, D6S113E, O27.1.1, BRD2-IT1, BRD2
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatibility complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.
Klonalität: Monoclonal
Molekulargewicht: 88kDa
NCBI: 6046
UniProt: P25440
Reinheit: Affinity purification
Target-Kategorie: BRD2
Application Verdünnung: WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human. ResearchArea: Epigenetics Nuclear Signaling,Signal Transduction,Kinase,Cell Biology,Developmental Biology,Ubiquitin. Shipping: Ice Bag
Western blot analysis of lysates from wild type (WT) and BRD2 knockdown (KD) 293T cells using [KD Validated] BRD2 Rabbit mAb (A23166) at 1:1000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.