Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:
Unconjugated
Alternative Synonym:
CD146, CD149, Muc18, s-endo, 1-gicerin, s-gicerin
Predicted to be involved in angiogenesis and positive regulation of cell migration. Predicted to act upstream of or within heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Predicted to be located in external side of plasma membrane, extracellular space, and nucleus. Predicted to be active in plasma membrane. Is expressed in several structures, including adipose tissue, genitourinary system, gut, nervous system, and respiratory system. Orthologous to human MCAM (melanoma cell adhesion molecule).
WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Human,Mouse. Shipping: Ice Bag
Western blot analysis of various lysates, using CD146 Rabbit pAb (A23669) at 1:800 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s.
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