TLE4 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A23693
Artikelname: TLE4 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A23693
Hersteller Artikelnummer: A23693
Alternativnummer: ABB-A23693-20UL,ABB-A23693-100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: ESG, BCE1, ESG4, GRG4, BCE-1, Grg-4, E(spI), E(spl), TLE4
Predicted to enable transcription corepressor activity. Predicted to be involved in negative regulation of canonical Wnt signaling pathway. Predicted to act upstream of or within Wnt signaling pathway, cellular response to leukemia inhibitory factor, and negative regulation of transcription by RNA polymerase II. Located in nucleoplasm. Part of beta-catenin-TCF complex.
Klonalität: Monoclonal
Molekulargewicht: 84kDa
NCBI: 7091
UniProt: Q04727
Reinheit: Affinity purification
Target-Kategorie: TLE4
Application Verdünnung: WB,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse. ResearchArea: Epigenetics Nuclear Signaling,Cell Biology Developmental Biology,Wnt -Catenin Signaling Pathway,Stem Cells. Shipping: Ice Bag
Western blot analysis of various lysates using TLE4 Rabbit mAb (A23693) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.