Enables endopeptidase inhibitor activity, proteasome binding activity, and thiol-dependent deubiquitinase. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process, positive regulation of smoothened signaling pathway, and protein deubiquitination. Located in cytosol, nucleolus, and nucleoplasm. Colocalizes with Ino80 complex.
Western blot analysis of various lysates, using UCH37/UCHL5 Rabbit mAb (A23792) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
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