ABflo 594 Rabbit IgG isotype control, Monoclonal

Artikelnummer: ABB-A23821
Artikelname: ABflo 594 Rabbit IgG isotype control, Monoclonal
Artikelnummer: ABB-A23821
Hersteller Artikelnummer: A23821
Alternativnummer: ABB-A23821-100T,ABB-A23821-2X100T,ABB-A23821-5X100T
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: ABflo 594
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells, by non-specific interactions with cellular proteins, carbohydrates, and lipids, or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Klonalität: Monoclonal
Konzentration: FC
Reinheit: Affinity purification
Application Verdünnung: FC,5 µl per 10 6 cells in 100 µl volume
Anwendungsbeschreibung: Shipping: Ice Bag
Flow cytometry: 1X10 6 HAP1 cells(negative control,left) and Daudi cells(right) were surface-stained with ABflo 594 Rabbit anti-Human CD352/SLAMF6 mAb(A23821,5 µl/Test,orange line) or ABflo 594 Rabbit IgG isotype control (A23821,5 µl/Test,blue line).Non-fluorescently stained cells were used as blank control (red line).