CYP11A1 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A23954
Artikelname: CYP11A1 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A23954
Hersteller Artikelnummer: A23954
Alternativnummer: ABB-A23954-100UL,ABB-A23954-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: CYP11A1, CYP11A, CYPXIA1, P450SCC, cytochrome P450 family 11 subfamily A member 1
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide.
Klonalität: Monoclonal
Molekulargewicht: 42kDa/60kDa
NCBI: 1583
UniProt: P05108
Reinheit: Affinity purification
Target-Kategorie: CYP11A1
Application Verdünnung: WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse. ResearchArea: Cancer,Signal Transduction,Cell Biology & Developmental Biology,Growth factor,Endocrine & Metabolism,Lipid Metabolism,Cholesterol Metabolism,Cytochromes,Lipases,Cardiovascular,Lipids. Shipping: Ice Bag
Western blot analysis of various lysates using CYP11A1 Rabbit mAb (A23954) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90 s.