PDK1/PDHK1 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A24749
- Bilder (1)
| Artikelname: | PDK1/PDHK1 Rabbit mAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A24749 |
| Hersteller Artikelnummer: | A24749 |
| Alternativnummer: | ABB-A24749-20UL,ABB-A24749-100UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | PDK1, pyruvate dehydrogenase kinase 1, PDK1/PDHK1 |
| Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene. |
| Application Verdünnung: | WB,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse. ResearchArea: Cancer,Signal Transduction,PI3K-Akt Signaling Pathway,Endocrine Metabolism,Carbohydrate metabolism,Warburg Effect,Immunology Inflammation,T Cell Receptor Signaling Pathway,NF-kB Signaling Pathway. Shipping: Ice Bag |
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Western blot analysis of various lysates using PDK1/PDHK1 Rabbit mAb (A24749) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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