[KD Validated] RGAP1 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A24948
Artikelname: [KD Validated] RGAP1 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A24948
Hersteller Artikelnummer: A24948
Alternativnummer: ABB-A24948-100UL,ABB-A24948-20UL,ABB-A24948-500UL,ABB-A24948-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: CYK4, CDAN3B, ID-GAP, HsCYK-4, MgcRacGAP, [KD Validated] RGAP1
This gene encodes a GTPase-activating protein (GAP) that is a compoment of the centralspindlin complex. This protein binds activated forms of Rho GTPases and stimulates GTP hydrolysis, which results in negative regulation of Rho-mediated signals. This protein plays a regulatory role in cytokinesis, cell growth, and differentiation. Alternatively spliced transcript variants have been found for this gene. There is a pseudogene for this gene on chromosome 12.
Klonalität: Monoclonal
Klon-Bezeichnung: [ARC57331]
Molekulargewicht: 71kDa
NCBI: 29127
UniProt: Q9H0H5
Reinheit: Affinity purification
Sequenz: SLPLEYWSQFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGNFFASPMLK
Target-Kategorie: RACGAP1
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:2000 - 1:12000|IHC-P,1:100 - 1:400|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Signal Transduction,G protein signaling,Small G proteins,Cell Biology Developmental Biology,Cell Cycle,Centrosome,Immunology Inflammation,Cytokines. Shipping: Ice Bag
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Western blot analysis of various lysates, using [KD Validated] RGAP1 Rabbit mAb (A24948) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human testis tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] RGAP1 Rabbit mAb (A24948) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.