CD38 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A25398
- Bilder (8)
| Artikelname: | CD38 Rabbit mAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A25398 |
| Hersteller Artikelnummer: | A25398 |
| Alternativnummer: | ABB-A25398-500UL,ABB-A25398-100UL,ABB-A25398-1000UL,ABB-A25398-20UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | ADPRC1, cADPR1, ADPRC 1 |
| The protein encoded by this gene is a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Crystal structure analysis demonstrates that the functional molecule is a dimer, with the central portion containing the catalytic site. It is used as a prognostic marker for patients with chronic lymphocytic leukemia. Alternative splicing results in multiple transcript variants. |
| Klonalität: | Monoclonal |
| Molekulargewicht: | 14 kDa/34 kDa |
| NCBI: | 952 |
| UniProt: | P28907 |
| Reinheit: | Affinity purification |
| Sequenz: | VPRWRQQWSGPGTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITEEDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYLADDLTWCGEFNTSKINYQSCPDWRKDCSNNPVSVFWKTVSRRFAEAACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGREDSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCT |
| Target-Kategorie: | CD38 |
| Application Verdünnung: | WB,1:1000 - 1:10000|IHC-P,1:500 - 1:2000|FC,1:500 - 1:1000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human. ResearchArea: Cancer,Cell Biology Developmental Biology,Apoptosis,Endocrine Metabolism,Immunology Inflammation,CDs,Stem Cells,Hematopoietic Progenitors. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embeddedHuman tonsil tissue usingCD38 Rabbit mAb(A25398) at a dilution of 1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman colon tissue usingCD38 Rabbit mAb(A25398) at a dilution of 1:800 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Western blot analysis of various lysates using CD38 Rabbit mAb (A25398) at 1:3000 dilutionincubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): Hep G2 Exposure time: 20 s. |
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Immunohistochemistry analysis of paraffin-embedded Cynomolgus monkey spleen tissue using CD38 Rabbit mAb (A25398) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Flow cytometry: 1X10 6 Hep G2 cells (negative control,left)and Daudi cells (right) were surface-stained with CD38 Rabbit mAb (A25398,2 µg/mL,orange line) or ABflo 647 Rabbit IgG isotype control (A22070,5 µl/Test,blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line). |
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Flow cytometry: 1X10 6 Daudi cells were surface-stained with ABflo 647 Rabbit IgG isotype control (A22070,5 µl/Test,left) or CD38 Rabbit mAb (A25398,2 µg/mL,right). |
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Flow cytometry: 1X10 6 Human PBMC were surface-stained with CD38 Rabbit mAb (A25398,2 µg/mL,orange line) or ABflo 647 Rabbit IgG isotype control (A22070,5 µl/Test,blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained Human PBMC were used as blank control (red line). |
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Flow cytometry: 1X10 6 Human PBMC were surface-stained with ABflo 647 Rabbit IgG isotype control (A22070,5 µl/Test,left) or CD38 Rabbit mAb (A25398,2 µg/mL,right). |
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