Ferritin Heavy Chain Rabbit PolymAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A25458PM
- Bilder (8)
| Artikelname: | Ferritin Heavy Chain Rabbit PolymAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A25458PM |
| Hersteller Artikelnummer: | A25458PM |
| Alternativnummer: | ABB-A25458PM-20UL,ABB-A25458PM-500UL,ABB-A25458PM-1000UL,ABB-A25458PM-100UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | FHC, FTH, HFE5, PLIF, FTHL6, PIG15, Ferritin Heavy Chain |
| This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. |
| Application Verdünnung: | WB,1:1000 - 1:6000|IF-P,1:50-1:200|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Signal Transduction,Endocrine Metabolism,Mitochondrial metabolism,Mitochondrial markers,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Cardiovascular,Blood,Serum Proteins. Shipping: Ice Bag |
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Western blot analysis of lysates from HT-29 cells using Ferritin Heavy Chain Rabbit PolymAb (A25458PM) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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Western blot analysis of various lysates using Ferritin Heavy Chain Rabbit PolymAb (A25458PM) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingFerritin Heavy Chain Rabbit PolymAb(A25458PM) at a dilution of1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedRat kidney tissue usingFerritin Heavy Chain Rabbit PolymAb(A25458PM) at a dilution of1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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Confocal imaging of paraffin-embedded Human breast cancer tissue using Ferritin Heavy Chain Rabbit PolymAb (A25458PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of paraffin-embedded Human kidney tissue using Ferritin Heavy Chain Rabbit PolymAb (A25458PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of paraffin-embedded Rat liver tissue using Ferritin Heavy Chain Rabbit PolymAb (A25458PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of paraffin-embedded Mouse liver tissue using Ferritin Heavy Chain Rabbit PolymAb (A25458PM, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x. |
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