CD66a/CEACAM1 Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	CD66a/CEACAM1 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A25475
Hersteller Artikelnummer:	A25475
Alternativnummer:	ABB-A25475-20UL,ABB-A25475-100UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, FC, IF, IHC-P, WB
Spezies Reaktivität:	Mouse
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	Bgp, Cc1, Hv2, Bgp1, Cea1, Cea7, Hv-2, MHVR, bb-1, C-CAM, CD66a, Cea-1, Cea-7, MHVR1, Mhv-1, mCEA1, mmCGM1, mmCGM2, mmCGM1a

Enables several functions, including signaling receptor binding activity, virion binding activity, and virus receptor activity. Involved in several processes, including common myeloid progenitor cell proliferation, granulocyte colony-stimulating factor signaling pathway, and negative regulation of macromolecule metabolic process. Acts upstream of or within several processes, including modulation by host of viral process, regulation of T cell activation, and regulation of intracellular signal transduction. Located in cell projection membrane, external side of plasma membrane, and extracellular space. Is expressed in several structures, including alimentary system, central nervous system, extraembryonic component, genitourinary system, and skeleton. Orthologous to several human genes including CEACAM1 (CEA cell adhesion molecule 1).

Klonalität:	Monoclonal
Molekulargewicht:	57kDa/50kDa/37kDa/30kDa
NCBI:	26365
UniProt:	P31809
Reinheit:	Affinity purification
Target-Kategorie:	Ceacam1

Application Verdünnung:	WB,1:5000 - 1:20000\|IF-P,1:200 - 1:800\|IHC-P,1:200 - 1:2000\|FC,1:500 - 1:1000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Mouse,Rat. ResearchArea: Labeling and Cell Labeling - Cell Type Labeling - Tumor Related. Shipping: Ice Bag

	Western blot analysis of lysates from Mouse liver usingCD66a/CEACAM1a Rabbit mAb (A25475) at1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time:30s.
	Immunohistochemistry analysis of paraffin-embeddedRat intestine tissue usingCD66a/CEACAM1a Rabbit mAb(A25475) at a dilution of1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embeddedMouse colon tissue usingCD66a/CEACAM1a Rabbit mAb(A25475) at a dilution of1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
	Confocal imaging ofparaffin-embedded Mouse liver tissue usingCD66a/CEACAM1a Rabbit mAb (A25475, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Confocal imaging ofparaffin-embedded Mouse small intestine tissue usingCD66a/CEACAM1a Rabbit mAb (A25475, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Confocal imaging ofparaffin-embedded Mouse spleen tissue usingCD66a/CEACAM1a Rabbit mAb (A25475, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
	Flow cytometry: 1X10 6 3T3-L1 cells (Low Expression,left)and C57BL/6 mouse Splenocytes (right) were surface-stained with CD66a/CEACAM1a Rabbit mAb (A25475,2 µg/mL,orange line) or PE Rabbit IgG isotype control (A24172,5 µl/Test,blue line), followed by PE conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
	Flow cytometry: 1X10 6 C57BL/6 mouse Splenocytes were surface-stained with PE Rabbit IgG isotype control (A24172,5 µl/Test,left) or CD66a/CEACAM1a Rabbit mAb (A25475,2 µg/mL,right).