Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:
Biotin
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells, by non-specific interactions with cellular proteins, carbohydrates, and lipids, or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Klonalität:
Monoclonal
Konzentration:
FC
Reinheit:
Affinity purification
Application Verdünnung:
FC,5 µl per 10 6 cells in 100 µl volume
Anwendungsbeschreibung:
Shipping: Ice Bag
Flow cytometry: 1X10 6 293T cells (negative control,left)and Jurkat cells (right) were surface-stained with Biotin Rabbit anti-Human CD45 mAb (A25627,5 µl/Test,orange line) or Biotin Rabbit IgG isotype control (A25626,5 µl/Test,blue line), followed by Alexa Fluor 488 Streptavidin staining (5 µl/Test,orange line). Non-fluorescently stained cells were used as blank control (red line).
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