[KD Validated] POLR2L Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A25872
- Bilder (8)
| Artikelname: | [KD Validated] POLR2L Rabbit mAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A25872 |
| Hersteller Artikelnummer: | A25872 |
| Alternativnummer: | ABB-A25872-20UL,ABB-A25872-500UL,ABB-A25872-1000UL,ABB-A25872-100UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-67 of human POLR2L (NP_066951.1). |
| Konjugation: | Unconjugated |
| Alternative Synonym: | RBP10, RPB10, RPABC5, RPB7.6, hRPB7.6, RPB10beta |
| This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases. |
| Application Verdünnung: | WB,1:2000 - 1:8000|IHC-P,1:1000 - 1:4000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Neuroscience,Neurodegenerative Diseases. Shipping: Ice Bag |
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Western blot analysis of lysates from 293T cells transfected with POLR2L siRNA using [KD Validated] POLR2L Rabbit mAb (A25872) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020) .Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Western blot analysis of various lysates using [KD Validated] POLR2L Rabbit mAb (A25872) at 1:2200 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s. |
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Immunohistochemistry analysis of paraffin-embedded Human cervix tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat skin tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] POLR2L Rabbit mAb (A25872) at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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