[KO Validated] CDK5 Rabbit mAb, Unconjugated, Monoclonal

Artikelname:	[KO Validated] CDK5 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A26254
Hersteller Artikelnummer:	A26254
Alternativnummer:	ABB-A26254-20UL,ABB-A26254-100UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	LIS7, PSSALRE

This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants.

Klonalität:	Monoclonal
Molekulargewicht:	33kDa
NCBI:	1020
UniProt:	Q00535
Reinheit:	Affinity purification
Target-Kategorie:	CDK5

Application Verdünnung:

WB,1:1000 - 1:4000|IF-P,1:200 - 1:800|IHC-P,1:200 - 1:800|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.

Anwendungsbeschreibung:

Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Cancer,Signal Transduction,G protein signaling,Kinase,Serine threonine kinases,Cell Biology Developmental Biology,Apoptosis,Cell Cycle,Endocrine Metabolism,Endocrine and metabolic diseases,Obesity,Immunology Inflammation,Cytokines,Neuroscience,Neurodegenerative Diseases,Amyloid Plaque and Neurofibrillary Tangle Formation in Alzheimers Disease,Dopamine Signaling in Parkinsons Disease,Neurodegenerative Diseases Markers. Shipping: Ice Bag

	Western blot analysis of lysates from wild type (WT) and CDK5 knockout (KO) 293T cells using CDK5 Rabbit mAb (A26254) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of various lysates using CDK5 Rabbit mAb (A26254) at 1:2000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KO Validated] CDK5 Rabbit mAb (A26254) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using [KO Validated] CDK5 Rabbit mAb (A26254) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] CDK5 Rabbit mAb (A26254) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] CDK5 Rabbit mAb (A26254) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of paraffin-embedded Mouse brain tissue using [KO Validated] CDK5 Rabbit mAb (A26254, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
	Confocal imaging of paraffin-embedded Rat brain tissue using [KO Validated] CDK5 Rabbit mAb (A26254, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.