CD66a/CEACAM1 Rabbit PolymAb, Unconjugated, Monoclonal

Artikelname:	CD66a/CEACAM1 Rabbit PolymAb, Unconjugated, Monoclonal
Artikelnummer:	ABB-A26413PM
Hersteller Artikelnummer:	A26413PM
Alternativnummer:	ABB-A26413PM-1000UL,ABB-A26413PM-20UL,ABB-A26413PM-500UL,ABB-A26413PM-100UL
Hersteller:	ABclonal
Wirt:	Rabbit
Kategorie:	Antikörper
Applikation:	ELISA, IF, IHC-P, WB
Spezies Reaktivität:	Human, Mouse
Immunogen:	Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation:	Unconjugated
Alternative Synonym:	Bgp, Cc1, Hv2, Bgp1, Cea1, Cea7, Hv-2, MHVR, bb-1, C-CAM, CD66a, Cea-1, Cea-7, MHVR1, Mhv-1, mCEA1, mmCGM1, mmCGM2, mmCGM1a

This gene encodes a member of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily. Two subgroups of the CEA family, the CEA cell adhesion molecules and the pregnancy-specific glycoproteins, are located within a 1.2 Mb cluster on the long arm of chromosome 19. Eleven pseudogenes of the CEA cell adhesion molecule subgroup are also found in the cluster. The encoded protein was originally described in bile ducts of liver as biliary glycoprotein. Subsequently, it was found to be a cell-cell adhesion molecule detected on leukocytes, epithelia, and endothelia. The encoded protein mediates cell adhesion via homophilic as well as heterophilic binding to other proteins of the subgroup. Multiple cellular activities have been attributed to the encoded protein, including roles in the differentiation and arrangement of tissue three-dimensional structure, angiogenesis, apoptosis, tumor suppression, metastasis, and the modulation of innate and adaptive immune responses. Multiple transcript variants encoding different isoforms have been reported, but the full-length nature of all variants has not been defined.

Klonalität:	Monoclonal
Molekulargewicht:	30kDa/37kDa/43kDa/46kDa/50kDa/57kDa
NCBI:	634
UniProt:	P13688
Reinheit:	Affinity purification
Target-Kategorie:	CEACAM1

Application Verdünnung:	WB,1:3000 - 1:12000\|IF/ICC,1:2000 - 1:8000\|IF-P,1:2000 - 1:8000\|IHC-P,1:4000 - 1:10000\|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:	Cross-Reactivity: Human,Mouse. ResearchArea: Immunology Inflammation,CDs,Cardiovascular,Angiogenesis. Shipping: Ice Bag

	Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using CD66a/CEACAM1 Rabbit PolymAb (A26413PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Western blot analysis of various lysates using CD66a/CEACAM1 Rabbit PolymAb (A26413PM) at 1:3000 dilutionincubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): HeLa Exposure time: 30s.
	Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD66a/CEACAM1 Rabbit PolymAb (A26413PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using CD66a/CEACAM1 Rabbit PolymAb (A26413PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using CD66a/CEACAM1 Rabbit PolymAb (A26413PM) at a dilution of 1:8000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
	Confocal imaging of Hep G2 cells using CD66a/CEACAM1 Rabbit PolymAb (A26413PM, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of SK-MEL-28 cells using CD66a/CEACAM1 Rabbit PolymAb (A26413PM, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
	Confocal imaging of paraffin-embedded Mouse intestine tissue using CD66a/CEACAM1 Rabbit PolymAb (A26413PM, dilution 1:4000) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.