CD3 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A26443
- Bilder (8)
| Artikelname: | CD3 Rabbit mAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A26443 |
| Hersteller Artikelnummer: | A26443 |
| Alternativnummer: | ABB-A26443-100UL,ABB-A26443-500UL,ABB-A26443-1000UL,ABB-A26443-20UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, FC, IF, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | T3E, TCRE, IMD18, CD3epsilon |
| The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. |
| Application Verdünnung: | WB,1:500 - 1:1000|IF-P,1:50 - 1:200|IHC-P,1:50 - 1:200|FC,1:100 - 1:500|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human. ResearchArea: Cell Biology Developmental Biology,Apoptosis,Immunology Inflammation,CDs,T Cell Receptor Signaling Pathway,Stem Cells,Hematopoietic Progenitors. Shipping: Ice Bag |
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Western blot analysis of various lysates using CD3 Rabbit mAb (A26443)at 1:1000 dilutionincubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): PC-3 Exposure time: 10s. |
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Confocal imaging of paraffin-embedded Human spleen tissue using CD3 Rabbit mAb (A26443, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x. |
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Confocal imaging of paraffin-embedded Human tonsil tissue using CD3 Rabbit mAb (A26443, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x. |
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Flow cytometry: 1X10 6 Raji cells (negative control,left) and Human PBMC (right) were surface-stained with CD3 Rabbit mAb (A26443,2 µg/mL,orange line) or Rabbit IgG isotype control (AC042,2 µg/mL,blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line). |
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Flow cytometry: 1X10 6 Human PBMC were surface-stained with Rabbit IgG isotype control (AC042,2 µg/mL,left) or CD3 Rabbit mAb (A26443,2 µg/mL,right), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. |
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Confocal imaging of paraffin-embedded Human tonsil tissue using CD3 Rabbit mAb (A26443, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x. |
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Flow cytometry: 1X10 6 Raji cells (negative control,left) and Human PBMC (right) were surface-stained with CD3 Rabbit mAb (A26443,2 µg/mL,orange line) or Rabbit IgG isotype control (AC042,2 µg/mL,blue line), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line). |
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Flow cytometry: 1X10 6 Human PBMC were surface-stained with Rabbit IgG isotype control (AC042,2 µg/mL,left) or CD3 Rabbit mAb (A26443,2 µg/mL,right), followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. |
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