TriMethyl-Histone H4-K20 Rabbit mAb, Unconjugated
Artikelnummer:
ABB-A27268
- Bilder (8)
| Artikelname: | TriMethyl-Histone H4-K20 Rabbit mAb, Unconjugated |
| Artikelnummer: | ABB-A27268 |
| Hersteller Artikelnummer: | A27268 |
| Alternativnummer: | ABB-A27268-500UL,ABB-A27268-20UL,ABB-A27268-1000UL,ABB-A27268-100UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | DOT, ELISA, IF, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | H4, H4/n, H4C1, H4C2, H4C3, H4C4, H4C5, H4C6, H4C8, H4C9, H4F2, H4FN, FO108, H4-16, H4C11, H4C12, H4C13, H4C15, H4C16, HIST2H4, HIST2H4A |
| Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated, this record represents the centromeric copy. |
| Application Verdünnung: | WB,1:16000 - 1:96000|DB,1:1000 - 1:2000|IHC-P,1:500 - 1:5000|IF/ICC,1:100 - 1:400|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted). ResearchArea: Epigenetics Nuclear Signaling. Shipping: Ice Bag |
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Western blot analysis of various lysates using TriMethyl-Histone H4-K20 Rabbit mAb (A27268) at 1:16000 dilutionincubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): H4 protein Exposure time: 20s. |
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Dot-blot analysis of all sorts of peptides using TriMethyl-Histone H4-K20 Rabbit mAb (A27268) at 1:1000 dilution. |
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Western blot analysis of various lysates using TriMethyl-Histone H4-K20 Rabbit mAb (A27268) at 1:16000 dilutionincubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): H4 protein Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using TriMethyl-Histone H4-K20 Rabbit mAb (A27268) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using TriMethyl-Histone H4-K20 Rabbit mAb (A27268) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using TriMethyl-Histone H4-K20 Rabbit mAb (A27268) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining. |
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Confocal imaging of NIH/3T3 cells using TriMethyl-Histone H4-K20 Rabbit mAb (A27268, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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Confocal imaging of HeLa cells using TriMethyl-Histone H4-K20 Rabbit mAb (A27268, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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