CD98/SLC3A2 Rabbit mAb, Unconjugated, Monoclonal

Artikelnummer: ABB-A28528
Artikelname: CD98/SLC3A2 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer: ABB-A28528
Hersteller Artikelnummer: A28528
Alternativnummer: ABB-A28528-500UL,ABB-A28528-1000UL,ABB-A28528-20UL,ABB-A28528-100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment). This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: 4F2, CD98, MDU1, 4F2HC, 4T2HC, NACAE, CD98HC
This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized.
Klonalität: Monoclonal
Molekulargewicht: 58-71 kDa
NCBI: 6520
UniProt: P08195
Reinheit: Affinity purification
Target-Kategorie: SLC3A2
Application Verdünnung: WB,1:2000-1:5000|IF-P,1:200-1:400|IHC-P,1:5000-1:20000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. For high-ratio antibody dilutions (1:10000),a sequential dilution meth
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,RNA Binding,Cancer,Signal Transduction,Endocrine Metabolism,Immunology Inflammation,CDs,Neuroscience,Calcium Signaling,Stem Cells,Hematopoietic Progenitors. Shipping: Ice Bag
Western blot analysis of various lysates using CD98/SLC3A2 Rabbit mAb (A28528) at 1:5000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45 s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using CD98/SLC3A2 Rabbit mAb (A28528) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using CD98/SLC3A2 Rabbit mAb (A28528) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas tissue using CD98/SLC3A2 Rabbit mAb (A28528) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using CD98/SLC3A2 Rabbit mAb (A28528) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using CD98/SLC3A2 Rabbit mAb (A28528) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Mouse kidney tissue using CD98/SLC3A2 Rabbit mAb (A28528, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat kidney tissue using CD98/SLC3A2 Rabbit mAb (A28528, dilution 1:200) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.