TTF-1 Rabbit mAb, Unconjugated, Monoclonal
Artikelnummer:
ABB-A3292
- Bilder (8)
| Artikelname: | TTF-1 Rabbit mAb, Unconjugated, Monoclonal |
| Artikelnummer: | ABB-A3292 |
| Hersteller Artikelnummer: | A3292 |
| Alternativnummer: | ABB-A3292-1000UL,ABB-A3292-20UL,ABB-A3292-100UL,ABB-A3292-500UL |
| Hersteller: | ABclonal |
| Wirt: | Rabbit |
| Kategorie: | Antikörper |
| Applikation: | ELISA, IF, IHC-P, WB |
| Spezies Reaktivität: | Human |
| Immunogen: | Synthetic peptide. This information is considered to be commercially sensitive. |
| Konjugation: | Unconjugated |
| Alternative Synonym: | BCH, BHC, NK-2, TEBP, TTF1, NKX2A, NMTC1, T/EBP, TITF1, TTF-1, NKX2.1, NKX2-1 |
| This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias TTF1 with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. |
| Klonalität: | Monoclonal |
| Klon-Bezeichnung: | [ARC1942] |
| Molekulargewicht: | 39kDa |
| NCBI: | 7080 |
| UniProt: | P43699 |
| Reinheit: | Affinity purification |
| Sequenz: | MSMSPKHTTPFSVSDILSPLEESYKKVGMEGGGLGAPLAAYRQGQAAPPTAAMQQHAVGHHGAVTAAYHMTAAGVPQLSHSAVGGYCNGNLGNMSELPPY |
| Target-Kategorie: | NKX2-1 |
| Antibody Type: | Primary Antibody |
| Application Verdünnung: | WB,1:1000 - 1:6000|IF-P,1:200 - 1:2000|IHC-P,1:500 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements. |
| Anwendungsbeschreibung: | Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors. Shipping: Ice Bag |
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Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using NKX2-1 Rabbit mAb (A3292) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Human lung tissue using NKX2-1 Rabbit mAb (A3292) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Western blot analysis of lysates from Mouse lung using TTF-1 Rabbit mAb (A3292) at 1:1000 dilution incubated overnight at 4°C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using NKX2-1 Rabbit mAb (A3292) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using NKX2-1 Rabbit mAb (A3292) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using NKX2-1 Rabbit mAb (A3292) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Confocal imaging ofparaffin-embedded Mouse thyroid usingNKX2-1 Rabbit mAb (A3292,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining. |
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Confocal imaging ofparaffin-embedded Human thyroid usingNKX2-1 Rabbit mAb (A3292,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining. |
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