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Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Desmin Rabbit mAb (A3736) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining. |
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Immunofluorescence analysis of paraffin-embedded mouse heart using Desmin Rabbit mAb (A3736) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Western blot analysis of various lysates using Desmin Rabbit mAb (A3736) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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Immunofluorescence analysis of paraffin-embedded rat heart using Desmin Rabbit mAb (A3736) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman thyroid tissue usingDesmin Rabbit mAb(A3736) at a dilution of 1:1600 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunohistochemistry analysis of paraffin-embeddedHuman lung tissue usingDesmin Rabbit mAb(A3736) at a dilution of 1:1600 (40x lens).High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining. |
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Immunofluorescence analysis of paraffin-embedded mouse heart using Desmin Rabbit mAb (A3736) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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Immunofluorescence analysis of paraffin-embedded rat heart using Desmin Rabbit mAb (A3736) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |