This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
WB,1:1000 - 1:10000|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Western blot analysis of various lysates using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at 1:1000 dilution. THP-1 cells were treated with PMA/TPA (80 nM) at 37°C for overnight or treated with LPS (1 µg/mL) at 37°C for 6 hours. Both NIH/3T3 cells and C6 cells were treated with TNF-alpha (20 ng/mL) at 37°C for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
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