Phospho-IkappaBalpha-S36 Rabbit mAb, Unconjugated

Artikelnummer: ABB-AP0999
Artikelname: Phospho-IkappaBalpha-S36 Rabbit mAb, Unconjugated
Artikelnummer: ABB-AP0999
Hersteller Artikelnummer: AP0999
Alternativnummer: ABB-AP0999-500UL,ABB-AP0999-1000UL,ABB-AP0999-20UL,ABB-AP0999-100UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IHC-P, WB
Spezies Reaktivität: Human
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: IKBA, MAD-3, NFKBI, EDAID2, Phospho-IkappaBalpha-S36
This gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease.
Klonalität: Monoclonal
Klon-Bezeichnung: [ARC1543]
Molekulargewicht: 36 kDa
NCBI: 4792
UniProt: P25963
Reinheit: Affinity purification
Sequenz: LDSMK
Target-Kategorie: NFKBIA
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:1000 - 1:10000|IHC-P,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat. ResearchArea: Epigenetics Nuclear Signaling,Transcription Factors,Protein phosphorylation,Cancer,Signal Transduction,MAPK-Erk Signaling Pathway,ATM Signaling Pathway,Cell Biology Developmental Biology,Apoptosis,Inhibition of Apoptosis,Death Receptor Signaling Pathway,Immunology Inflammation,B Cell Receptor Signaling Pathway,T Cell Receptor Signaling Pathway,NF-kB Signaling Pathway,Toll-like Receptor Signaling Pathway,Cell Intrinsic Innate Immunity Signaling Pathway,TLR Signaling. Shipping: Ice Bag
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Western blot analysis of various lysates using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at 1:1000 dilution. THP-1 cells were treated with PMA/TPA (80 nM) at 37°C for overnight or treated with LPS (1 µg/mL) at 37°C for 6 hours. Both NIH/3T3 cells and C6 cells were treated with TNF-alpha (20 ng/mL) at 37°C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human spleen using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse intestin using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse liver using Phospho-IkappaBalpha-S36 Rabbit mAb (AP0999) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.