Trinitrophenol was used as the immunogen for this Isotype control.
A Rat isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific Rat antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[2A3]
Reinheit:
Affinity Chromatography
Formulierung:
Purified
Antibody Type:
Isotype Control
Application Verdünnung:
FACS: 0.5-2 µg/10 6 cells
Fig.1: Cell Surface staining of splenocytes using 0.5 µgof Rat IgG2a isotype control. Goat anti-Rat FITC secondary antibody was used. Purple: cells alone, Green: rat IgG2a isotype control (10-106).
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