Interleukin-3 receptor subunit alpha (IL-3RA) or CD123 is a high-affinity receptor for interleukin-3 (IL-3), which along with the primary signaling commonbeta(deltac) subunit (CD131) forms a heterodimer, enabling cell signaling. IL-3RA chain is a glycoprotein of 360 amino acidic residues, composed by an extracellular domain of 287 amino acid residues, involving a predicted Ig-like domain, two Fn III domains, a transmembrane domain of 30 amino acid residues and by an intracellular domain of 53 residues. It is expressed on the majority of CD34+ hemopoietic progenitors and its expression is rapidly lost during erythroid and megakaryocytic differentiation, moderately decreased during monocytic differentiation and is sustained in the granulocytic lineage. IL-3RA is also expressed in a variety of hematological neoplasms including blastic plasmacytoid dendritic cell neoplasm (BPDCN), acute myeloid leukemia (AML), acute lymphoblastic leukemia (ALL), chronic myeloid leukemia (CML), Hodgkin-s lymphoma and hairy cell leukemia.
Fig-1: Western blot analysis of IL-3RA. Anti-IL-3RA antibody (Clone: ABM25C1) was used at 6 µg/ml on hbrain lysate.
Fig-2: Cell surface flow analysis of human IL3-RA in PBMCs (lymphocytes gated) using 0.5 µg/10 6 cells of antibody (Clone: ABM25C1). Green represents isotype control, red represents anti-IL3 RA antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were incubated with primary antibody for 45 min. then washed twice with PBS by centrifuging at 1100 rpm for 5 min, followed by 30 min incubation with conjugated secondary antibody. Data acquisition was done after washing twice w
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