Interleukin-2-dependent human T lymphocytes were used as the immunogen for the CD45RO antibody.
Alternative Synonym:
PTPRC||CD45
The CD45RA and CD45RO isoforms of the leukocyte common antigen identify functionally distinct naive and memory T cell subsets. In vitro, CD45RA+ / CD45RO - peripheral blood lymphocytes (PBL) can be converted to CD45RA- / CD45RO + phenotype upon activation in the presence of IL-2. Both CD3+ and CD3-/CD56+ lymphocyte subsets can be converted to CD45RO + lymphocytes. Expression of CD45RO was observed only in response to IL-2 and was not observed during long-term culture in IL-4, IL-6, or IL-7. This antibody recognizes an 180kDa protein, identified as isoform of leukocyte common antigen (CD45RO). This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. Shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Though this antibody is useful to identify T-cell lymphomas and leukemia, rare staining with B cell lymphomas reported.
Fig.1: Cell Surface flow analysis of hCD45RO in PBMC (Lymphocytes gated) using 0.5 µg antibody per10 6 cells. Green represents isotype control (ABEOMICS), red represents anti-hCD45RO antibody (10-7509). Goat anti-mouse PE conjugated secondary antibody was used (ABEOMICS).
Fig: 2 Immunohistochemical analysis of CD45RO in human tonsil using CD45RO antibody (Clone: C45.1) at 1:500 dilution.
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