This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-alphaa), Heparin-binding EGF (HB-EGF), amphiregulin, beta cellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT)(Digest formalin-fixed tissues with Protease (Sigma Cat. P-6911) at 1mg/ml PBS, pH 7.4 for 10 min at 37C)
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