Monoclonal Antibody to Actin, Muscle Specific (Muscle Cell Marker)(Clone : MSA/953), Clone: [MSA/953], Mouse
Artikelnummer:
ABI-36-11080
Hersteller Artikelnummer:
36-11080
Alternativnummer:
ABI-36-11080-100UG
Hersteller:
Abeomics
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC, WB
Spezies Reaktivität:
Human, Rat
Immunogen:
Synthetic peptide common among human muscle actins
Alternative Synonym:
ACTA2||ACTSA||ACTVS||GIG46
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Western Blot (0.5-1.0ug/ml),,Immunohistochemistry (Formalin-fixed) (0.5-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 &degC followed by cooling at
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