Epitope of GZ-1 and GZ-20 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.
Flow Cytometry (5-10ul/million cells), Immunofluorescence (1:50-1:100), Western Blot (1:100-1:200), Immunoprecipitation (5-10ul/500ug protein lysate),Immunohistochemistry (Frozen & formalin-fixed) (1:100-1:200 for 30 min at RT)(Formalin/paraffin tissu
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