It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[BRD469 + BRD494 + BRD.3]
Reinheit:
Affinity Chromatography
Formulierung:
Purified
Target-Kategorie:
Bromodeoxyuridine (BrdU) (Proliferation Marker)
Application Verdünnung:
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Use Ab at 1ug/ml for 30 minute at RT)[For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min
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