Neuraminidase treated GLS-1 human small cell lung carcinoma cells
Alternative Synonym:
EPCAM||GA733-2||M1S2||M4S1||MIC18||TACSTD1||TROP1
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Flow Cytometry (5-10ul/million cells), Immunofluorescence (1:50-1:100), Western Blot (1:100-1:200), Immunohistochemistry (Formalin-fixed) (1:100-1:200 for 30 min at RT)(Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsi
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