Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker)(Clone : GP1.4), Clone: [GP1.4], Mouse
Artikelnummer:
ABI-36-1447
Hersteller Artikelnummer:
36-1447
Alternativnummer:
ABI-36-1447-100UG
Hersteller:
Abeomics
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
IHC, WB
Spezies Reaktivität:
Human
Immunogen:
Human milk fat globule membranes
Alternative Synonym:
MUC1||PUM
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Western Blot (1-2ug/ml), ,Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),
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