Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker)(Clone : SPM533), Clone: [SPM533], Mouse
Artikelnummer:
ABI-36-1450
Hersteller Artikelnummer:
36-1450
Alternativnummer:
ABI-36-1450-100UG
Hersteller:
Abeomics
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
FACS, IF, IHC
Spezies Reaktivität:
Human
Immunogen:
Delipidated extract of human milk fat globule membranes
Alternative Synonym:
MUC1||PUM
In Western blotting, it recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit and the C-terminal beta subunit. . The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinomas. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Flow Cytometry (1-2ug/million cells), Immunofluorescence (1-2ug/ml), Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)((Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min
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