Produced in Balb/c mice using highly pure recombinant Human Adiponectin as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS.
Antibody Type:
Monoclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 µg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.. Sandwich: In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 µg/ml.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
