Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEG-VEGF. Anti-Human EG-VEGF specific antibody was purified by affinity chromatography employing immobilized hVEGF matrix.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect hEG-VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEG-VEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect hEG-VEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human EG-VEGF (60-117BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEG-VEGF.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
