Produced from sera of rabbits pre-immunized with highly pure recombinant Human FGF-acidic. Anti-Human FGF-acidic specific antibody was purified by affinity chromatography employing immobilized Human FGF-acidic matrix.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect Human FGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human FGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect Human FGF-acidic by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Human FGF-acidic (60-132BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human FGF-acidic.. Immunohistochemistry: <span style=font-family: verdana, font-size: 13px,>This antibody stained formalin-fixed, paraffin-embedded sections of human normal kidney. The recommended concentration is 0.050 µg/mL-0.125 µg/mL with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. <br /> . Neutralization: To yield one-half maximal inhibition [ND<span style=font-size: 16px,>50] of the biological activity of Human FGF-acidic (10.0 ng/ml), a concentration of 1.0-2.5 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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