Produced from sera of rabbits pre-immunized with highly pure recombinant Rat IFN-gamma. Anti-Rat IFN-gamma specific antibody was purified by affinity chromatography employing immobilized Rat IFN-gamma matrix.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect Rat IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IFN-gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect Rat IFN-gamma by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Rat IFN-gamma (62-061BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IFN-gamma.. Immunohistochemistry: This antibody stained colchicine injected rat brain (including the cortex and median eminence) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA(TM)) reagent. Optimal concentrations and conditions may vary. . Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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