Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1alpha. Anti-Murine IL-1alpha specific antibody was purified by affinity chromatography employing immobilized mIL-1alpha matrix.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect mIL-1alpha by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems Biotinylated Anti-Murine IL-1alpha (61-001ABT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1alpha.. Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA(TM)) reagent. Optimal concentrations and conditions may vary. . Neutralization: To yield one-half maximal inhibition [ND<span style=font-size: 16px,>50] of the biological activity of mIL-1alpha (0.05 ng/ml), a concentration of 0.0 3- 0.05 µg/ml of this antibody is required.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
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