Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-31. Anti-Human IL-31 specific antibody was purified by affinity chromatography and then biotinylated.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect hIL-31 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-31 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect hIL-31 by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems Polyclonal Anti-Human IL-31 (60-031P) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31.. Direct: To detect hIL-31 by direct ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-31.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
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