Produced from sera of rabbits immunized with highly pure recombinant Murine IL-5. Anti-Murine IL-5 specific antibody was purified by affinity chromatography and then biotinylated.
Formulierung:
A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect Murine IL-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents, the detection limit for recombinant Murine IL-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. <span style=font-size: 12pt, font-family: times new roman, serif,>. Sandwich: To detect Murine IL-5 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems Polyclonal Anti- Murine IL-5 (61-005P) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5.<span style=font-size: 12pt, font-family: times new roman, serif,>. Direct: To detect Murine IL-5 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant Murine IL-5.. Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
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