Produced from sera of rabbits immunized with highly pure recombinant Rat IL-7. Anti-Rat IL-7 specific antibody was purified by affinity chromatography and then biotinylated.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems Polyclonal Anti-Rat IL-7 ( ) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-7.. Direct: To detect Rat IL-7 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-7.
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