<span id=ctl00_SPWebPartManager1_g_2c64f2a8_025c_4a21_8444_332def32454d_ctl00_lblPreparationText>Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hKlotho. Anti-Human Klotho specific antibody was purified by affinity chromatography and then biotinylated.
Antibody Type:
Polyclonal Antibody
Anwendungsbeschreibung:
WesternBlot: To detect hKlotho by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKlotho is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.. Sandwich: <span id=ctl00_SPWebPartManager1_g_2c64f2a8_025c_4a21_8444_332def32454d_ctl00_lblSandwichText>To detect hKlotho by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems Polyclonal Anti-Human Klotho () as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hKlotho.. Direct: <span id=ctl00_SPWebPartManager1_g_2c64f2a8_025c_4a21_8444_332def32454d_ctl00_lblELISAText>To detect hKlotho by direct ELISA (using 100 µl/well antibody solution) a concentration of 0.25 - 1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hKlotho.
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